Engineered Human Antibody Constant Domains with Increased Stability
نویسندگان
چکیده
منابع مشابه
Shortened engineered human antibody CH2 domains: increased stability and binding to the human neonatal Fc receptor.
The immunoglobulin (Ig) constant CH2 domain is critical for antibody effector functions. Isolated CH2 domains are promising scaffolds for construction of libraries containing diverse binders that could also confer some effector functions. We have shown previously that an isolated human CH2 domain is relatively unstable to thermally induced unfolding, but its stability can be improved by enginee...
متن کاملShortened Engineered Human Antibody Ch2 Domains: Increased Stability and Binding to the Human Neonatal Receptor
The immunoglobulin (Ig) constant CH2 domain is critical for antibody effector functions. Isolated CH2 domains are promising scaffolds for construction of libraries containing diverse binders that could also confer some effector functions. We have previously shown (Gong et al., JBC 2009) that an isolated human CH2 domain is relatively unstable to thermally induced unfolding but its stability can...
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We have created mouse-human antibody molecules of defined antigen-binding specificity by taking the variable region genes of a mouse antibody-producing myeloma cell line with known antigen-binding specificity and joining them to human immunoglobulin constant region genes using recombinant DNA techniques. Chimeric genes were constructed that utilized the rearranged and expressed antigen-binding ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2009
ISSN: 0021-9258
DOI: 10.1074/jbc.m900769200